Spore Strips (Chem Vapor / Steam)

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Geobacillus stearothermophilus (ATCC 7953) – For use in Chem Vapor / Steam sterilization at 121°C to 135°C. After exposure to sterilant, incubate strips at 55-60°C.

Kayospore Biological Indicator Spore Strips consists of bacterial spores of Geobacillus Stearothermophilus ATCC 7953 inoculated onto a paper after filter carrier contained within a medical grade paper for use in Chem Vapor/Steam sterilization. The spore strip is removed from the load and aseptically transferred into appropriate microbiological culture medium.

MINIMUM POPULATION: 1.0 X 106

PACK SIZE: One Pack of 100 Nos.

SPORE CARRIER: 6.5mm X 38mm

SHELF LIFE: 18 months

Instructions for use :

  • Place indicators in the areas of the pack or load least accessible to steam. Places such as the geometrical center, and upper and lower regions of both front and rear of the loas to be sterilized are considered suitable for placement of these indicators.
  • On completion of sterilization cycle , spore strip is removed from the load and aseptically transferred into appropriate microbiological culture medium. Using sterile scissors, cut open end envelope. Thereafter remove the indicator with Sterile tweezers and aseptically transfer it to a tube of Sterile Soyabean Casein Digest Medium.
  • A standard procedure should be established for the routine evaluation of each sterilizer.

Precautions : 

  • Kayospore Biological Indicator Spore Strips Cultures of Geobacillus Stearothermophilus  must be autoclaved at 121° C for at least 30 minutes prior to discarding.


Activation, Incubation and Result :

Number of spores present per strip: 10, these organism are difficult to destroy because they are more resistant to  heat than other vegetative bacteria and viruses. Therefore, if they are destroyed during sterilization, it is assumed  that all other life forms are also destroyed. So transfer each spore strips into a tube containing Soybean Casein Digest Medium. 

This tubes should be placed in the incubator immediately after the strips are cultured. Their placement in an optimized growth environment is necessary to gain accurate results.

Incubate at 55-60° for 7 days, regularly checking for spore growth. Results should be read no later than 7 days after incubation.

Clear medium indicates that the spores were killed in the sterilization process. The appearance of turbidity indicates Bacterial Growth (Positive). Any positive result, should be reported immediately to a supervisor and sterilizers taken out of service until resolved.

Environmental Limitations : Storage: 60-80° F, 30-70 % RH, away from sterilizing agents, avoid Sunlight and all other forms of UV light.

Available Options

Specifications

Minimum population 1.0 X 106
Pack size One Pack of 100 Nos
Shelf life 18 months
Spore Carrier 6.5mm X 38mm Strip
Use Chem Vapor / Steam
Spore Geobacillus stearothermophilus

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